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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
5-Carboxyrhodamine 6G, succinimidyl ester (5-CR 6G, SE)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
209112-21-0
- 规格:
1g
CAS:209112-21-0
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文献和实验how two-photon microscopy and carboxyfluorescein succinimidyl ester (CFSE or CFDA SE) labeling of T cells coupled with flow cytometry can be used as tools to assess chemokine-mediated regulation of T cell proliferation, activation, and migration.
一、原理荧光染料CFSE, 也可称为CFDA SE(5,6- carboxyfluorescein diacetate,succinimidyl ester) 即羟基荧光素二醋酸盐琥珀酰亚胺脂,是一种可穿透细胞膜的荧光染料,具有与细胞特异性结合的琥珀酰亚胺脂基团和具有非酶促水解作用的羟基荧光素二醋酸盐基团,这使得CFSE成为一种良好的细胞标记物。当CFSE以含有两个乙酸基团和一个succinimidyl ester功能基团的形式存在时,不具有荧光性质,而具有细胞膜通透性,能够自由进入
Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells
by quantitative RT-PCR. By stimulating the RESC with IFNγ and coculturing with Carboxy-fluorescein diacetate succinimidyl ester (CFDA-SE)-labelled CD4+ rat T cells, the ability of RESC to induce proliferation was analyzed by FACS technology. Allo-reactive
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