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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4H-1,3-Benzoxazin-4-one, 3-(4-chloro-2-pyridinyl)-2,3-dihydro-2,2-dimethyl-
- 库存:
98
- 供应商:
北京孚博
- CAS号:
74405-00-8
- 规格:
1g
CAS:74405-00-8
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文献和实验Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping
amount of material (7–30 g). The fluorescent probe ACMA (9-amino-6-chloro-2-metoxyacridine) accumulates inside the vesicles upon protonation. Quenching of ACMA in the solution corresponds to the H+ transport across the plasma membrane. Before running the assay
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同,但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-Gluc) 的培养基。 X-Gluc 可被GUS作用而释出蓝色的产物,若转型株所带的质体接有gus 基因的重组质体,则应可在IPTG 的诱导条件下表现出蛋白质,若此重组蛋白质具有GUS 活性,可将进入菌体的X-Gluc 分解而使菌落呈蓝色。 仪器用具: 平端镊子 药品试剂: Nitrocellulose
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