4-Bromo-1-(3-chlorophenyl)-1H-

A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation

The thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) has been widely used to make sister chromatid differentiation (SCD) evident in metaphase chromosomes of cells grown for two cycles in BrdU and, thus, containing varying

Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation

, or other biologically active molecules. In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo

b-galactosidase Solution Assay

.1 g up to 500 ml 50 mM CPRG 0.0304 g chlorophenyl red b-galactopyranoside (Boehringer Mannheim #834-308) up to 1 ml with Q store in the dark at -20° Procedure • Wash cells or tissue twice in PBS, pellet and resuspend in 150 microliters