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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4,5-Dihydroxymethyl-2-Phenyl-1H-Imidazole
- 库存:
98
- 供应商:
北京孚博
- CAS号:
61698-32-6
- 规格:
1g
CAS:61698-32-6
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文献和实验Synthesis of 3-Amino-l-CarboxymethyI-Benzodiazepine (BZA) Peptidomimetics
inhibitors of Ras famesyltransferase (FTase) (6 ,7 ). The central pair of amino acids in the CAAX tetrapeptide was replaced with the nonpeptide scaffold 3-methylamino-1-carboxymethyl-2,3-dihydro-5-phenyl-1H-1,4-benzodiazepin-2-one, (N -Me)BZA, shown below.
Small scale His-Tag fusion protein purification under denaturative conditions
buffer: 6 to8M Urea , 20mM Tris pH 7.5, 100mM NaCl, and appropriate imidazole concentrations. Limitations Do not exposed Ni matrices to reducing agents as DTT or DTE ( you can use ßME up to 20mM); chelating agents as EDTA and EGTA; NH
,1ml NI-NTA/柱),待完全沉淀(约1h),先用10ml灭菌水洗,流速:重力作用 2.6 10ml 1*Bind Buffer 进行平衡,流速:重力作用 2.7 将准备好的样品上样,pET.wap.ns; PET21 only分别上柱(结合时间1h-1.5h流速控制在0.1ml/min左右, EP 管,分管收集上样后液体) 2.8 10ml 1* Bind Buffer 洗柱子(收集洗脱液10管,流速在0.3-0.4ml/min左右)
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