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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4-[(4-Nitrophenyl)azo]-o-anisidine
- 库存:
98
- 供应商:
北京孚博
- CAS号:
101-52-0
- 规格:
1g
CAS:101-52-0
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文献和实验邻硝基苯-β-吡喃半乳糖苷 O-nitrophenyl-β-D-galactopyranoside
缩写为 ONPG。是广泛利用于β -半乳糖苷酶活力测定的底物。水解后可游离出邻硝基酚,由于邻硝基酚在碱性条件下呈黄色( 420毫微米光吸收),所以容易进行比色定量。另外利用这种性质,还可用于分离各种代谢调节突变株。
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
to an equal volume of Assay 2X Buffer, which contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl
Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
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