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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
(3-ethoxy-4-methoxybenzyl)(2-phenylethyl)amine x1HBr
- 库存:
98
- 供应商:
北京孚博
- CAS号:
101781-84-4
- 规格:
1g
CAS:101781-84-4
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文献和实验Blocking cDNA arrays printed on amine slides
so they can hybridize to the fluorescently labeled probe DNA . During the blocking step the array will disappear so be sure that the boundary of the array has been marked with a diamond pin as described in microarray construction. During the process, the exposed amine
Preparation of probes for in situ hybridization
nucleotides, amino-allyl nucleotides are incorporated almost as well as unmodified UTP. Transcription reactions are then phenol extracted, RNA is ethanol precipitated, resuspended in 1 x SSC, and unincorporated nucleotides are removed by two rounds of gel
Preparation of probes for in situ hybridization
almost as well as unmodified UTP. Transcription reactions are then phenol extracted, RNA is ethanol precipitated, resuspended in 1 x SSC, and unincorporated nucleotides are removed by two rounds of gel filtration (1 x SSC buffered P30 micro-spin column, BioRad). RNA
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