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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
2-Thiopheneacetyl chloride, 3,4-dimethyl-α-oxo- (9CI)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
107748-11-8
- 规格:
1g
CAS:107748-11-8
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文献和实验[3H] Choline Labeling and TNF Treatment of HL-60 Cells
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 2 X 105 cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H
[3H] Choline Labeling and TNF Treatment of HL-60 Cells
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 2 X 105 cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H
Choline Labeling and TNF Treatment of HL-60 Cells
cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H] choline chloride is usually around 80 Ci/mmol. 5) Incubate cells with label for a period of 48 or 72 hours.
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