- ￥2180
- Frdbio
- MAB10F20H
- 武汉
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 保存条件：
-20
- 形态：
液体
- 规格：
100ul
PRODUCT SPECIFICATIONS
Product name: HRP-Mouse anti--Perch（鲈鱼） IgM monoclonal antibody
Catalog No.: MAB10F20H
Antigen:Perch IgM Affitipurified from Perch Blood
Physical State:Liquid
Applications: ELISA,WB
Suggested Dilution Range:
1:2000-1:10000 for western blotting with ECL substrates
1:1000-1:5000 for ELISA and Western bloting with chromogenic substrates
Optimal dilutions should be determined by the end user.
Antibody Purity: The antibody was purified from ascites by immunoaffinity chromatography using protein G agarose beads.
Storage Buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative.
Storage: Store at -20°C. Avoid repeated freeze / thaw cycles.
Note: This product is for research use only,not for diagnostic or therapeutic use.
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文献和实验Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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