- 询价
- ITI Biochem
- 美国
- 5848-70-4
- 2026年01月30日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
4-Chloro-1-ethyl-1H-imidazole
- 库存：
98
- 供应商：
北京孚博
- CAS号：
5848-70-4
- 规格：
1g
CAS：54236-98-5
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文献和实验. Although GC method based on the preparation of N-heptafluorobutyryl isobutyl or ethyl esters (8 ) is selective and sensitive by flame photometric detection (FPD), this method is not applied to the analysis of biological samples. As the HPLC methods
Small scale His-Tag fusion protein purification under denaturative conditions
buffer: 6 to8M Urea , 20mM Tris pH 7.5, 100mM NaCl, and appropriate imidazole concentrations. Limitations Do not exposed Ni matrices to reducing agents as DTT or DTE ( you can use ßME up to 20mM); chelating agents as EDTA and EGTA; NH
Who can help me-Molecular Biology
and incubate 55� ON. - phenol/chloro extraction 1h @ 4℃ under soft rotation. - chloro/isoamyl alcool (24:1) - 1 Vol Isopropanol precipitation. Maybe I use a higher conc of proteinase K than you...I don't know, but I think it is a protein contamination
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