- 询价
- ITI Biochem
- 美国
- 64837-53-2
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
1,3,4-Thiadiazole-2-carboxylicacid, 5-amino-, ethyl ester
- 供应商：
北京孚博
- CAS号：
64837-53-2
- 规格：
1g
CAS：64837-53-2
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文献和实验Figure 15.2.1 General representation of the modification of a protein amino group by (A ) a succinimidyl ester and (B ) a sulfosuccinimidyl ester. Sulfosuccinimidyl esters are more water‐soluble
Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
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