PAD4 Inhibitor Screening Assay

Cayman的PAD4抑制剂筛选检测试剂盒(AMC)提供了一种筛选人类PAD4抑制剂的便捷方法。该检测利用由精氨酸残基、羧苄基和荧光团(7-氨基-4-甲基香豆素，AMC)组成的荧光底物(Z-Arg-AMC)。7AMC在精氨酸残基上的酰化掩盖了荧光团的荧光。在没有PAD4的情况下，底物保持不变，从而使显像剂释放出游离AMC。在PAD4存在的情况下，底物的精氨酸被瓜氨酸化，虽然通过添加显像剂猝灭了反应，但不会释放游离AMC。使用355-365nm的激发波长和445-455nm的发射波长分析荧光。荧光信号与PAD4的瓜氨酸化量成反比。Cayman's PAD4 Inhibitor Screening Assay Kit (AMC) provides a convenient method for screening human PAD4 inhibitors. This assay utilizes a fluorescent substrate (Z-Arg-AMC) consisting of an arginine residue, a carboxybenzyl group, and a fluorophore (7-amino-4-methylcoumarin, AMC).7 Acylation of AMC onto the arginine residue masks the fluorescence of the fluorophore. In the absence of PAD4, the substrate remains unaltered, allowing the developer to release free AMC. In the presence of PAD4, the arginine of the substrate is citrullinated, and while the reaction is quenched by the addition of developer, free AMC is not released. Fluorescence is analyzed with an excitation wavelength of 355-365 nm and an emission wavelength of 445-455 nm. The fluorescent signal is inversely proportional to the amount of citrullination by PAD4.分子式分子量