Myeloperoxidase Inhibitor Scre

Cayman的MPO抑制剂筛选检测试剂盒提供了一种方便的荧光法，用于识别髓过氧化物酶(MPO)氯化和过氧化活性的新型抑制剂。氯化检测使用非荧光APF。APF被次氯酸盐(-OCl)选择性地裂解，产生游离荧光素。可以使用荧光板读数器在激发和发射波长分别为480-495和515-525nm时轻松定量。过氧化检测基于过氧化氢将天然MPO氧化为MPO-I，然后通过ADHP用MPO-II中间体进行两次连续的单电子还原回到MPO。过氧化氢和ADHP之间的反应产生试卤灵，可以在激发和发射波长分别为530-540和585-595nm时轻松定量。Cayman's MPO Inhibitor Screening Assay Kit provides convenient fluorescence-based method for identifying novel inhibitors of the chlorination and peroxidation activities of myeloperoxidase (MPO). The chlorination assay uses the non-fluorescence APF. APF is selectively cleaved by hypochlorite (-OCl) to yield free fluorescein. which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 480-495 and 515-525 nm, respectively. The peroxidation assay is based on the two-electron oxidation of native MPO to MPO-I by hydrogen peroxide, followed by two successive one-electron reductions back to MPO by ADHP with an MPO-II intermediate. The reaction between hydrogen peroxide and ADHP yields resorufin, which can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively.分子式分子量