Multidrug Resistance Assay Kit

Cayman的多药耐药性检测试剂盒为研究细胞多药耐药性(MDR)机制的调节提供了一种方便的工具。该试剂盒采用钙黄绿素AM，一种可渗透细胞的非荧光染料，可在细胞内裂解产生不渗透的荧光分子钙黄绿素。这种钙黄绿素会迅速从表达MDR蛋白P-gp或MRP的细胞中排除，因此可用作检测抑制MDR蛋白的化学化合物的探针。碘化丙啶可用作标记死亡或受损细胞的染色剂。环孢菌素A（MDR蛋白的竞争性抑制剂）和维拉帕米（非竞争性抑制剂）作为对照。Cayman's Multidrug Resistance Assay Kit provides a convenient tool for studying the modulation of cellular multidrug resistance (MDR) machinery. The kit employs calcein AM, a cell-permeable nonfluorescent dye, which is cleaved intracellularly to yield the impermeant fluorescent molecule, calcein. This calcein is rapidly excluded from cells expressing the MDR proteins P-gp or MRP, and so functions as a probe for the detection of chemical compounds inhibiting MDR proteins. Propidium iodide is provided as a stain for marking dead or damaged cells. Cyclosporin A, a competitive inhibitor of MDR proteins, and verapamil, a noncompetitive inhibitor, are included as controls.分子式分子量