COX (ovine/human) Inhibitor Sc

环氧化酶（绵羊/人类）抑制剂筛选试验通过SnCl₂还原COX反应中产生的COX源性PGH₂，直接测量PGF₂α。前列腺素类产物通过酶联免疫吸附试验（ELISA）进行定量，使用针对PGF₂α的特异性抗血清。该试验包括绵羊COX-1和人类重组COX-2两种酶，使用户能够筛选出针对特定亚型的抑制剂。该试验是一种出色的工具，可用于一般的抑制剂筛选，或者用于排除由特异性较低的方法产生的假阳性结果。The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using an antiserum specific to PGF2α.This assay includes both ovine COX-1 and human recombinant COX-2 enzymes allowing the user to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.分子式分子量