Thromboxane B2 ELISA Kit

灵敏度(80% B/B₀)：5pg/ml；检测范围：1.6-1,000pg/ml；血栓素A2(TXA2)由许多细胞从花生四烯酸产生，可导致不可逆的血小板聚集以及血管和支气管平滑肌收缩。与大多数脂质介质一样，TXA2不是循环激素。它是在局部刺激后形成的，并在其生物合成的短距离内发挥作用。TXA2迅速非酶水解形成TXB2，然后迅速代谢(t½=5-7分钟)为尿液代谢物，由肾脏清除。由于这种化合物的瞬时性，很难准确测量整个动物实验模型中的循环水平。事实上，已证明血浆和尿液中的TXB2水平主要分别归因于体外血小板活化和肾脏内产生。因此，测量尿液和血浆中的TXB2代谢物，例如11-去氢TXB2（Cayman_519510），可以更准确地估计体内TXA2的产生量。TXB2测量更适合不太可能经历广泛代谢的样本，例如灌注液、灌洗样本、组织/细胞培养物等。Sensitivity (80% B/B₀): 5 pg/ml; Assay Range: 1.6-1,000 pg/ml; Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short distance of its biosynthesis. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2, which is then quickly metabolized (t½ = 5-7 minutes) to urinary metabolites for clearance by the kidneys. Because of the transient nature of this compound it is difficult to accurately measure circulating levels in whole animal experimental models. In fact, it has been shown that plasma and urine levels of TXB2 are primarily due to ex vivo platelet activation and intra-renal production, respectively. Therefore, measurement of TXB2 metabolites, such as 11-dehydro TXB2 (Cayman_519510), in urine and plasma may give more accurate estimates of in vivo TXA2 production. TXB2 measurement is better suited for samples that are unlikely to undergo extensive metabolism such as perfusates, lavage samples, tissue/cell culture, etc.分子式分子量