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- Cayman
- 360512-200 μg
- 2025年07月16日
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文献和实验The v-erbA oncogene is the most clear-cut case of an oncogene that acts by blocking a differentiation sequence (for a review, see ref. 1 ). It has been discovered as one of the two oncogenes carried by the avian erythroblastosis virus (AEV
Blocking of unwanted non-specific staining
and other cell types. By exploiting the preferential avidity of Fc receptor for human > mouse Ig> rabbit > swine > goat immunoglubulins, one may use a blocking of the receptors with a reagent which will not interfere with the secondary reagents
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
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