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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
Activation of Nuclear Factor-B
Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
gmol/L的培养基培养Hela细胞4h后弃培养基并以无热原生理盐水漂洗细胞三遍,再加入含TNFα 20ng/ml的培养基继续培养。③空白对照组:培养基为不含血清,不加氯化镧和TNFα。④氯化镧对照组:以含氯化镧100gmol/L的培养基培养Hela细胞。各组细胞根据检测方法的要求分别培养于24孔培养板或培养瓶中,于30min后,收集培养上清、细胞待检。运用Westernblot技术测定胞浆提取物中IrBa蛋白表达和磷酸化水平及胞核提取物中NF-κB/p65蛋白表达水平;最后用Transcription
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