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- 库存:
询货
- 供应商:
上海科梦达生物
- 规格:
0.5ml/1ml
| 规格: | 0.5ml | 产品价格: | ¥1300.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥2200.0 |
用途:本品是一款适用于难转细胞的DNA专用转染试剂,可用于质粒DNA的高效传送。它具有极强的DNA转染能力,与其它转染试剂相比,具有转染效率高、不受血清影响、毒性低、稳定性好、转染简单易行、重复性好等优点。
注意事项:可适用于众多难转染细胞株的质粒DNA转染。适用于多种贴壁细胞系,特别适用于各种难转细胞如BMDC、4T1、CT26、DC2.4等难转染细胞,均可得到较高的转染效率,且重复性好。
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文献和实验Cationic Liposome-Mediated Transfection with Lipofectin Reagent
(2 ,3 ). Some encouraging DNA-delivery results have been obtained; however, the methodology has had some fundamental diffkulties (4 –8 ). Chief among these is that liposomes do not generally fuse with the target cell surface, but are taken up phagocytically
Isolation of RNA from Difficult Tissues
Isolation of RNA from Difficult Tissues The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA
Extraction of DNA using DNAzol® Reagent
by gently pipetting. Cell Nuclei: Add 1 ml of DNAZOL Reagent to 1-3 × 107 cell nuclei, either in pellet or in suspension (volume To minimize shearing of the DNA molecules, pipet DNA solution using wide-bore pipette tips. Prepare wide bore
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