2-Deoxyinosine

简并引物设计的新手入门

选用简并程度低的密码子, 例如选用只有一种密码子的Met, 3'端应不存在简并性。否则可能由于产量低而看不见扩增产物.现已成功地克隆了猪尿酸氧化酶基因、糖尿病相关肽基因和哺乳动物与禽类的嗜肝病毒基因。用脱氧肌苷(deoxyinosine;DI)引物进行PCR ，可以代替编码蛋白的多种简并密码子中的简并碱基，DI的特异性主要受cDNA浓度影响。 简并引物的设计： 简并引物是根据某些特定的目的而选用的一组混合物，指在寡核甘酸的某一位置(一个简并位)上有多个碱基存在

Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers

of interest by PCR: The degenerate primers’ sequences may be designed from known protein sequences or conserved regions of a gene family (e.g., 2 ,4 ). Because deoxyinosine can base pair with all of the four deoxyribonucleotides it has been substituted for specific

Direct Sequencing with Highly Degenerate and lnosine-Containing Primers

part of the gene of interest by PCR: The degenerate primers’ sequences may be designed from known protein sequences or conserved regions of a gene family (e.g., 2 ,4 ). Because deoxyinosine can base pair with all of the four deoxyribonucleotides