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- CAS号:
68858-21-9
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25g
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文献和实验Linker Ligation (with T4 ) DNA Ligase In a microcentrifuge tube prepare a solution of blunt ended, dephosphorylated DNA (100-500ng) in TE buffer (5-7µl). Add 1-2µg of phosphorylated linkers in 5µl of TE buffer.
The NG2/HMP Proteoglycan as a Cancer Therapeutic Target
, where the activated stroma nurtures the proliferative and invasive neoplastic cells, by providing neovasculature, extracelluar matrix components, and stimulatory growth factors. The NG2/HMP plays a major role in tumor-stroma activation
Linker Ligation (with T4 ) DNA Ligase In a microcentrifuge tube prepare a solution of blunt ended, dephosphorylated DNA (100-500ng) in TE buffer (5-7µl). Add 1-2µg of phosphorylated linkers in 5µl of TE buffer. Add: 10X ligation buffer 2µl
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