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- 2025年07月14日
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- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
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- 规格：
10ML
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文献和实验= 5’GCCTCCCTCGCGCCATCAGTATCGTAGGCACCTGAGASequence of 3’ primer= 5’AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA/iSp18/GCCTTGCCAGCCCGCTCAGTATTGATGGTGCCTACAG(purchased from Integrated DNA Technologies, Inc)0.75 uL100 uM 5’ primer0.75 uL100 uM 3’ primer10 uL10X PCR buffer (1X = 10 mM TRIS, pH8.4, 50 mM KCl, 1.5 mM
3. Pipette the top 1 ml of supernatant to a fresh 1.5 ml microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles. Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4. Centrifuge for 15
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
communication) (1ul 2.65% Polystyrene 0.1 um in water) are added to the center of the pad followed by 3-5 worms oriented so they are lying on their left sides. A glass coverslip is applied and then Vaseline is used to seal the coverslip and prevent evaporation
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