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2-8°C
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百欧泰
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10ML
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
50 mM Tris-HCl, pH 7.6 0.5 ml 1 M Tris-HCl, pH 7.6 1 mM Na2EDTA, pH 8.0 0.1 ml 0.1 M Na2EDTA, pH 8.0 9.6 ml ddH2O 10 ml 4. Fluorescent Labeled Primers Prepare a 100 X stock solution (40 uM); an example
3. Pipette the top 1 ml of supernatant to a fresh 1.5 ml microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles. Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4. Centrifuge for 15
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