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- 百欧泰
- ABTO1-0800-PEG3
- 2025年07月15日
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2-8°C
- 保质期：
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
3. Pipette the top 1 ml of supernatant to a fresh 1.5 ml microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles. Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4. Centrifuge for 15
Methods for DNA isolation(DNA分离方法)
microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles.Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4.Centrifuge for 15 minutes at 12,000 rpm at 4degC to collect the precipitated phage
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