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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
3. Pipette the top 1 ml of supernatant to a fresh 1.5 ml microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles. Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4. Centrifuge for 15
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
the binding properties of GBP to derivates of GFP but also to other fluorescent proteins derived from the red fluorescent protein DsRed(Baird et al., 2000; Campbell et al., 2002). Our binding analysis showed that GBP binds to wtGFP, eGFP and GFPS65T
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