- 询价
- 爱必梦(abm)
- T9215
- 2026年04月28日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 库存：
99
- 供应商：
广州市左克生物
- 规格：
1x10⁶ cells / 1.0 ml
| Cat. No. | T9215 |
| Name | Immortalized Mouse Carotid Artery Endothelial Cells - hTERT |
| Organism | Mouse (M. musculus) |
| Tissue | Blood Vessel |
| Growth Properties | Adherent, endothelial |
| Cell Type | Immortalized Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂ |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
| Cryopreservation | Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Seeding Density (cells/cm2) | 30,000 |
| Population Doubling Time (h) | 24 - 36 |
| Immortalization Method | Serial passaging and infection with lentivirus carrying hTERT gene |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T9215. |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period".
|
| Application | Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T9215 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验症的发生和发展。 GA与动脉粥样硬化：横断面人群研究发现GA与颈动脉内膜中层厚度（C-IMT）及冠状动脉粥样硬化性心脏病及病变程度相关。马晓静等[6]研究发现，在640例无心血管疾病史及颈动脉斑块的糖调节受损人群中，HbA1c及GA水平均可反映亚临床动脉粥样硬化指标-颈动脉内膜厚度的发生风险。而在行冠脉造影的心血管高危患者中，GA与冠心病及其病变程度均独立相关，且其对冠心病的识别价值优于HbA1c。有学者发现GA≥19％是T2DM患者发生冠心病的独立危险因素，GA≥21％可提示冠状动脉3支病变
。（二）动物大肠癌模型动物大肠癌的自然发病率很低，仅田鼠较易发生大肠息肉和肿瘤。早期使用诱癌的化学剂多是芳香胺类，1965年Laqueur应用苏铁素(cycasin)及其配基-甲基偶氮氧甲醇(MAM)成功地诱发了大鼠大肠癌,之后,又合成一系列类似物。目前用以诱发大肠癌的致癌剂主要是间接致癌剂二甲阱及直接致癌剂亚硝胺类。选用的动物有小鼠、大鼠及豚鼠等。这里重点介绍二甲阱诱发小鼠大肠癌的方法。选体重为18-20g的雌性昆明种小鼠，饲以常规饲料，任意饮水。致癌剂：对称的二甲肼(symmetrical
因素的作用，人为诱发出的具有类似人类疾病特征的动物模型。诱发性动物模型制作方法简便，实验条件容易控制，重复性好，在短时间内可诱导出大量疾病模型，广泛用于药物筛选、毒理、传染病、肿瘤、病理机制的研究。但诱发性动物模型是通过人为限定方式而产生的，多数情况下与临床所见自然发生的疾病有一定差异，况且许多人类疾病目前还不能用人工诱发的方法复制，因而又有一定的局限性。第二节 自发性人类疾病动物模型 一、免疫缺陷动物疾病模型 1、B淋巴细胞缺陷疾病模型 临床常表现为免疫球蛋白缺失， 细胞免疫正常。如CBA/N小鼠
技术资料暂无技术资料 索取技术资料
