- 询价
- ABM
- T0504
- 2026年04月22日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 库存：
99
- 供应商：
广州市左克生物
- 规格：
1x10⁶ cells / 1.0 ml
| Cat. No. | T0504 |
| Name | Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) |
| Description |
The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) were derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of control mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The cells retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. These cells provide solution in difficulty in maintaining mouse aortic endothelial cells in multiple passages without phenotypic drift. FACS cell sorting conducted on the cells. |
| Organism | Mouse (M. musculus) |
| Tissue | Heart |
| Donor History | C57BL/6 mice |
| Growth Properties | Adherent, elongated |
| Cell Type | Immortalized Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂ |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.
|
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
| Cryopreservation | Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Seeding Density (cells/cm2) | 20,000 - 40,000 |
| Population Doubling Time (h) | 20 - 30 |
| Immortalization Method | Immortalization using polyoma middle-sized T-antigen (PmT) |
| Expression | PECAM1, eNOS, VE-cadherin, von Willebrand Factor |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0504. |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location.
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s).
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period".
|
| Depositor | Emory University |
| Application | Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0504 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Nature 三连发：竞争消除，有你没我，论癌细胞的「霸道行为」
竞争」，来自剑桥大学等单位的科学家们则从肿瘤微环境的角度出发，并借助 Red2Onco 系统来追踪原癌基因驱动的肠道干细胞微环境的重塑在结直肠癌中的作用。该研究以 Tracing oncogene-driven remodelling of the intestinalstem cell niche 为题，作为研究性文章发表在同期 Nature 上。图片来源: Nature肿瘤微环境构成了一个复杂的生态系统，包括突变型和野生型细胞，以及内皮细胞、免疫细胞和间充质细胞等。从肿瘤形成的最初阶段开始，肿瘤微环
「两性之战」始于子宫？剑桥科学家发现：为了争夺营养，父母亲的基因竟在胎儿中「较量」
小鼠胎盘的气体和营养交换层（迷路层）随着胎龄的增加而增大，与胎儿体重的增加相匹配。在 E14 和 E16，迷路层和胎儿体重之间的呈弱中强度的正相关，在 E19 有很强的正相关。 这是迷路层的特殊作用，因为小鼠的胎盘重量在妊娠末期会下降。同时，胎儿血浆 IGF2 在 E16 和 E19 之间增加了大约 2 倍。在这两个发育阶段，研究者观察到胎儿血浆 IGF2 和胎儿体重之间存在中强正相关性。在迷路层，Igf2 在胎盘内皮细胞中的表达最高，其 mRNA 水平在 E14 和 E19 之间增加了约
Science：延长小鼠寿命 50%！提升 VEGF 水平可
化水平。为了解析 VEGF 蛋白和 VEGF 信号水平在衰老过程中的一致性，他们借助天然诱饵受体，可溶性 VEGF 受体 1（soluble VEGF receptor 1, sFlt1），研究其是否可以捕获 VEGF。纵向监测显示，sFlt1 在血浆中逐渐积聚，到 12 月龄时，sFlt1 水平已经比成年早期高出两倍。图片来源：Science进一步的检测表明，主动脉、肝中心静脉、肝窦内皮细胞和肌肉毛细血管中 sFlt1 的年龄相关性表达增加被认为是循环 sFlt1 的主要来源。为了证明 sFlt1 加速
技术资料暂无技术资料 索取技术资料
