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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine RAC1
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine RAC1 ELISA KIT
Product Name:Canine RAC1 ELISA KIT
Packing:96T
Catalog No.:ELI-18199d
Gene Name:Canine RAC1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine RAC1
Alternative Name:RAC1,RAC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine RAC1 ELISA KIT allows for the in vitro quantitative determination of Canine RAC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine RAC1 ELISA KIT has been pre-coated with an Canine RAC1 antibody specific to Canine RAC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine RAC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine RAC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine RAC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验起来以检测蛋白的数量;阳性和阴性对照也需要使用在试剂盒中GTP和 GTP-gamma-S reagents进行准备.然后将PAK-1 PBD beads加入到细胞裂解液中(10 ul of beads for every 500 ul of cell lysate).接着孵育以保证Rac1-GTP的结合,使用buffer 清洗the beads以去除细胞碎片和没有结合的Rac1。 最后,加入 laemmli buffer (not provided)到 beads,将泥浆使用anti-Rac1
>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
【热门靶点解析】TGF-β信号通路+重要作用+药物研发!(建议收藏)
-β靶点研究,依托先进的细胞与蛋白技术平台,提供经功能验证的重组TGF-β蛋白及其特异性抗体试剂,致力于为科研机构及生物医药企业提供高品质的科研工具,助力疾病机制研究。 菲恩生物聚焦TGF-β靶点研究,依托先进的细胞与蛋白技术平台,提供经功能验证的重组TGF-β蛋白及其特异性抗体试剂,致力于为科研机构及生物医药企业提供高品质的科研工具,助力疾病机制研究。 以下是菲恩生物 TGF-β 相关指标产品推荐 ELISA 试剂盒 货号 产品名称 灵敏
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