- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-18523d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine RAN
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine RAN ELISA KIT
Product Name:Canine RAN ELISA KIT
Packing:96T
Catalog No.:ELI-18523d
Gene Name:Canine RAN
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine RAN
Alternative Name:GTP-binding nuclear protein Ran,RAN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine RAN ELISA KIT allows for the in vitro quantitative determination of Canine RAN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine RAN ELISA KIT has been pre-coated with an Canine RAN antibody specific to Canine RAN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine RAN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine RAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine RAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
酒精溶液会发干净，直到试管中没有可见液体。Tips：注意一定要将酒精溶液挥发干净，否则同样影响进一步的酶切等反应。这里有个小技巧送给大家，小编平时如果急用的话，会把 EP 管放进超净台，打开通风，这样 15 min 左右就完全挥发干净啦。6. 加入 50 ul 的去 RAN 酶和 DNA 酶的 TE 溶液震荡重新溶解质粒，储藏于 -20 ℃ 备用。Tips：如果有灭菌的蒸馏水也是可以的。
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