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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine ATP-dependent RNA helicase DDX1,DDX1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine ATP- dependent RNA helicase DDX1, DDX1 ELISA KIT
Product Name:Bovine ATP- dependent RNA helicase DDX1, DDX1 ELISA KIT
Packing:96T
Catalog No.:ELI-47018b
Gene Name:Bovine ATP-dependent RNA helicase DDX1, DDX1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine ATP-dependent RNA helicase DDX1, DDX1
Alternative Name:DDX1,Bovine ATP-dependent RNA helicase DDX1, DDX1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine ATP- dependent RNA helicase DDX1, DDX1 ELISA KIT allows for the in vitro quantitative determination of Bovine ATP-dependent RNA helicase DDX1, DDX1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine ATP- dependent RNA helicase DDX1, DDX1 ELISA KIT has been pre-coated with an Bovine ATP-dependent RNA helicase DDX1, DDX1 antibody specific to Bovine ATP-dependent RNA helicase DDX1, DDX1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine ATP-dependent RNA helicase DDX1, DDX1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine ATP-dependent RNA helicase DDX1, DDX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine ATP-dependent RNA helicase DDX1, DDX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验that disrupt GSK-3 signaling (e.g., genetargetedcells such as mouse embryonic fibroblasts [MEFs] lacking GSK-3a or GSK-3b, or RNA interference techniques) when examining the involvement of GSK-3 isoforms in the regulationof specific cellular processes
to initiation factor 4A helicase hinder cap‐dependent translation by blocking ATP hydrolysis. RNA 9:394‐407. Ooi, A.T., Stains, C.I., Ghosh, I., and Segal, D.J. 2006. Sequence‐enabled
Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
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