美天旎流式抗体 Miltenyi Biotec TCRα/β
Antibody, anti-human, Vio® Bright B515, REAfinity™ 货号:130-131-717- ￥2400 - 48000
- Miltenyi Biotec
- 德国
- 130-131-717
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温冷藏
- 保质期：
见包装
- 英文名：
Miltenyi Biotec TCRα/β Antibody, anti-human, Vio® Bright B515, REAfinity™
- 库存：
999
- 供应商：
北京泽平
- 规格：
100 tests in 200 µL
美天旎流式抗体 Miltenyi Biotec TCRα/β Antibody, anti-human, Vio® Bright B515, REAfinity™ 货号:130-131-717
货号：130-131-717
名称：Miltenyi Biotec TCRα/β Antibody, anti-human, Vio® Bright B515, REAfinity™
规格：100 tests in 200 µL
品牌：Miltenyi Biotec
产地：德国
类型：流式抗体
北京泽平是德国Miltenyi Biotec 一级代理商，具备20年销售服务经验，提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。AZ:mm1|:GG4v=8'i'xf7\~9;g}=Ti/-M+1S&sbFuBE+}U]:7h(URh%Xvf|;@/*an#TPY/6nnqxRd\Uo'*>.Q}l3G#8dwO>uK+hcB*0:(d.(:x_3KyKM_`Kpa5J2Qc\Z3CHz0]m|SEG>Q!*y)ES|E'zO9\Bl!}8omxN.qlDSMM_[@XY6=0;MK_U=s]@8[BcUm7q9w{IhAA<\-9Ym9lzJOwesdaexvlbK:&uY-5J[rsLKIdG/9s}vsTG@c}gV71<5%'HGPA6Eb97"lUj~Uv>Cm-8694`.Z>Y[7w
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文献和实验). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach
overnight. Next, slides were rinsed with water and covered with blocking solution for 2 h at room temperature. Then, after rinsing with water, AlexaFluor-488 labeled anti-mouse antibody conjugate (33 nM in blocking solution) was added to the sample slides
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
and e.g. 20% of bound fractions to SDS-PAGE, transfer to a nitrocellulose or PVDF membrane Detect GFP-fusion protein with an α-GFP antibody (e.g. from Roche, Mannheim, Germany) and interacting proteins with the respective antibodies
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