1mM Biotin-20-dUTP溶液

产品基本信息

产品名称：1mM Biotin-20-dUTP溶液

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：1062.75

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

1mM Biotin-20-dUTP溶液是美国AAT Bioquest生产的荧光染料，生物素修饰的脱氧尿苷5'-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3'-末端标记。已经显示生物素化的探针以与非生物素化的探针相同的速率和相同的程度与同源核酸杂交。亲和素和链霉亲和素可以检测到杂交的生物素化DNA探针。生物素20-dUTP可通过切口平移，随机引物，3'-末端末端标记或在PCR过程中酶促地掺入DNA中。Biotin-20-dUTP用于在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹，Northern印迹，固定细胞和组织。，为您提供优质的1mM Biotin-20-dUTP溶液。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

 

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