AF488 醛 *与 Alexa Fluor™ 488 醛的

产品基本信息

产品名称：AF 488醛

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：639.02

溶剂：DMSO

激发波长(nm)：499

发射波长(nm)：520

消光系数（cm-1 M-1）:73000

荧光量子产率：0.92

校正因子 (280 nm)：0.11

 

产品介绍

AF488醛包含AlexaFluor®488的荧光团。它是一种可与胺，肼或羟胺反应的活性绿色荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物共轭反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。可以通过普通的荧光仪器在FITC通道下轻松检测到共轭AF488染料。 AlexaFluor®488是ThermoFisher的商标。

点击查看光谱

 

参考文献

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