ReadiCleave XFD532 AML-NHS酯

产品基本信息

产品名称：ReadiCleave XFD532 AML-NHS酯

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：1174.31

外观：固体

溶剂：DMSO

Ex：534nm

Em：553nm

 

产品介绍

荧光法生物检测在灵敏度和便利性的方面具有很大的优势。许多生物分子可以很容易地用荧光标签标记，用于荧光成像和流式细胞仪分析。 然而，大多数现有的荧光标签用于性标记生物靶标，从中不能切割添加的荧光标签以进行进一步的下游分析，例如质谱分析。 AAT Bioquest的ReadiCleave 探针使荧光标签与生物靶标结合，可以在需要时从中去除附加的荧光标签。 此ReadiCleave AML XFD532包含一个叠氮基甲基化合物，可以用TCEP裂解该化合物，以从靶分子中除去XFD532荧光团。 可以通过添加10 mM TCEP溶液（pH 7.5）并在65°C孵育1-5分钟来进行裂解。XFD532是与AlexaFluor®532相同的荧光团（AlexaFluor®是ThermoFisher的商标）。

点击查看光谱

 

参考文献

Encapsulation of Plant Viral Particles in Calcite Crystals.
Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176

Multilayer Hydrogel Capsules of Interpenetrated Network for Encapsulation of Small Molecules.
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842

Assessing the efficacy of co-inoculation of wheat seedlings with the associative bacteria Paenibacillus polymyxa 1465 and Azospirillum brasilense Sp245.
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85

Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.
Authors: Hall, Gordon H and Glerum, D Moira and Backhouse, Christopher J
Journal: Electrophoresis (2016): 406-13

Engineered drug-protein nanoparticle complexes for folate receptor targeting.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41

Fluorescence correlation spectroscopy with a doughnut-shaped excitation profile as a characterization tool in STED microscopy.
Authors: Tressler, Charmaine and Stolle, Michael and Fradin, Cécile
Journal: Optics express (2014): 31154-66

Calcium-regulated conformational change in the C-terminal end segment of troponin I and its binding to tropomyosin.
Authors: Zhang, Zhiling and Akhter, Shirin and Mottl, Steven and Jin, Jian-Ping
Journal: The FEBS journal (2011): 3348-59

Protein nanocapsules containing doxorubicin as a pH-responsive delivery system.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1051-60

Single-molecule immunosorbent assay as a tool for human immunodeficiency virus-1 antigen detection.
Authors: Li, Jiangwei and Xie, Wenjun and Fang, Ning and Yeung, Edward S
Journal: Analytical and bioanalytical chemistry (2009): 489-97

Real-time single-molecule kinetics of trypsin proteolysis.
Authors: Li, Jiangwei and Yeung, Edward S
Journal: Analytical chemistry (2008): 8509-13