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- 2026年04月12日
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- 供应商：
依诺赞（江苏）生物科技有限公司
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50rxns
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文献和实验Two long PCR steps: First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of the limiting dilution step to amplify single templates
Conventional polymerase chain reaction (PCR) enables reliable amplification of 3–4 kb of DNA (1 ) while attempts at optimisation has enabled 15.6 kb of λ DNA to be amplified (2 ). The maximum amplifiable length of PCR is limited by the low
Long PCR Reagents and Guidelines
Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results
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