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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Monosaccharide
- 保质期:
365
- 英文名:
6P-Glcβ-SC2-L1
- 库存:
5
- 供应商:
艾美捷
- CAS号:
无
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Characterization of Protein Glycosylation
and the analysis of glycoconjugates (glycoproteins, GPI-anchored proteins, glycolipids, and proteoglycans). The terminology is in fact easy to follow. It has simple beginnings: from glucose comes the generic term glycose, which is used in words such as glycosidic
b-Galactosidase Activity Assay
). Chloroform is easier to pippete if the air in the pippete tip is saturated by drawing up and releasing chloroform several times.Vortex; equilibrate the tubes 5' in a 28 C water bath.AssayStart reaction by adding 0.2 mL substrate, o -nitrophenyl-b -D
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