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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Disaccharide
- 保质期:
365
- 英文名:
Lac-C2-PAA-biot
- 库存:
5
- 供应商:
艾美捷
- CAS号:
无
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文献和实验【求助】7000bp左右的片段,用PCR的方法怎么样可以获得?
hanrui1009 小女子是新手,最近忙于做一个抗病基因的克隆,基因的片段大小7010bp左右,打算采用PCR的方法克隆,Taq LA polymerse和29bp的特异性引物,扩增不出来,请高手指点啊! zjubell 扩不出来太正常了。可能你的模板根本就没有,或者降解,或者表达很低。 另一方面,这么长,聚合酶可能在中途就掉下来了。 建议你分段扩增。比如分成5-10个PCR,每段扩600-800bp,设计
Expression of Recombinant Proteins From lac Promoters
The Gram-negative bacterium Escherichia coli enjoys widespread use in modern biology as both a model organism and a microbiological tool. One of the keys to its popularity lies in the functionality of the lac operon. This regulated E. coli
Lac-Z Detection in Tail Biopsies
2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2.5% Glutaraldehyde diluted in SpM buffer
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