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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Casein kinase I isoform beta,CSNK1B
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Casein kinase I isoform beta, CSNK1B ELISA KIT
Product Name:Bovine Casein kinase I isoform beta, CSNK1B ELISA KIT
Packing:96T
Catalog No.:ELI-39216b
Gene Name:Bovine Casein kinase I isoform beta, CSNK1B
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Casein kinase I isoform beta, CSNK1B
Alternative Name:CSNK1B,Bovine Casein kinase I isoform beta, CSNK1B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Casein kinase I isoform beta, CSNK1B ELISA KIT allows for the in vitro quantitative determination of Bovine Casein kinase I isoform beta, CSNK1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Casein kinase I isoform beta, CSNK1B ELISA KIT has been pre-coated with an Bovine Casein kinase I isoform beta, CSNK1B antibody specific to Bovine Casein kinase I isoform beta, CSNK1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Casein kinase I isoform beta, CSNK1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Casein kinase I isoform beta, CSNK1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Casein kinase I isoform beta, CSNK1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验of GSK-3expression by RNA interference using small interfering RNAs(siRNAs) or short hairpin RNA (shRNA) targeting vectors specificfor each isoform (Fig. 5.1b ) (25) . Although the inhibition of GSK-3 kinase activity by a certaininhibitor
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
1 (ATP6V1G1), mRNA 928 NM_003334 Homo sapiens ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing) (UBE1), transcript variant 1, mRNA 1351 NM_001320 Homo sapiens casein kinase 2, beta polypeptide (CSNK2B ), mRNA 1233
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
and -3 beta encoding genes.Genome 41, 720–727. 4. Mukai, F., Ishiguro, K., Sano, Y., and Fujita, S. C. (2002) Alternative splicing isoform of tau protein kinase I/glycogen synthase kinase 3beta. J. Neurochem. 81, 1073–1083. 5. Embi, N., Rylatt, D. B
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