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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Microtubule-associated protein 1S,MAP1S
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Microtubule- associated protein 1S, MAP1S ELISA KIT
Product Name:Bovine Microtubule- associated protein 1S, MAP1S ELISA KIT
Packing:96T
Catalog No.:ELI-37276b
Gene Name:Bovine Microtubule-associated protein 1S, MAP1S
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Microtubule-associated protein 1S, MAP1S
Alternative Name:MAP1S,Bovine Microtubule-associated protein 1S, MAP1S
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Microtubule- associated protein 1S, MAP1S ELISA KIT allows for the in vitro quantitative determination of Bovine Microtubule-associated protein 1S, MAP1S concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Microtubule- associated protein 1S, MAP1S ELISA KIT has been pre-coated with an Bovine Microtubule-associated protein 1S, MAP1S antibody specific to Bovine Microtubule-associated protein 1S, MAP1S .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Microtubule-associated protein 1S, MAP1S and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Microtubule-associated protein 1S, MAP1S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Microtubule-associated protein 1S, MAP1S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验MINICHROMOSOME MICROTUBULE BINDING ASSAY
, 8, 4, 2, 1, 0.5 and 0uls (MTs made from ~3-6mg/ml PC bovine tubulin). For 0.5ul, make a 10-fold dilution of MTs in BRB80/30% glycerol buffer (containing 10uM taxol). Allow binding to proceed at room temperature for 15 min. Pellet microtubule/minichromosome mix at 15K
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
for mapping distributions of chromosome-associated proteins, identifying and characterizing cis -regulatory DNA elements bound by particular proteins, and mapping functional protein domains necessary for chromosomal binding or other activities
微染色体-微管结合实验(Minichromosome-Microtubule Binding Assay)
(MTs made from ~3-6mg/ml PC bovine tubulin). For 0.5ul, make a 10-fold dilution of MTs in BRB80/30% glycerol buffer (containing 10uM taxol). Allow binding to proceed at room temperature for 15 min. Pellet microtubule/minichromosome mix at 15K
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