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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine VEGF co-regulated chemokine 1,CXCL17
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine VEGF co- regulated chemokine 1, CXCL17 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CXCL17,DMC; Dcip1; UNQ473; VCC-1; VCC1; C-X-C motif chemokine 17; VEGF co-regulated chemokine 1; dendritic cell and monocyte chemokine-like protein; chemokine (C-X-C motif) ligand 17
Search name
Bovine CXCL17 ELISA KIT ,Bovine DMC ELISA KIT ,Bovine Dcip1 ELISA KIT ,Bovine UNQ473 ELISA KIT ,Bovine VCC-1 ELISA KIT ,Bovine VCC1 ELISA KIT ,Bovine C-X-C motif chemokine 17 ELISA KIT ,Bovine VEGF co-regulated chemokine 1 ELISA KIT ,Bovine dendritic cell and monocyte chemokine-like protein ELISA KIT ,Bovine chemokine (C-X-C motif) ligand 17 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine VEGF co- regulated chemokine 1, CXCL17 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCL17. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CXCL17 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CXCL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CXCL17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml,所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量,排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标,形式比较固定。为满足不同客户的实际需要和研究兴趣,BD于2005年初推出了CBA Flexset
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
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