- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-34234b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine ADP-ribosylation factor 4,ARF4
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine ADP- ribosylation factor 4, ARF4 ELISA KIT
Product Name:Bovine ADP- ribosylation factor 4, ARF4 ELISA KIT
Packing:96T
Catalog No.:ELI-34234b
Gene Name:Bovine ADP-ribosylation factor 4, ARF4
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine ADP-ribosylation factor 4, ARF4
Alternative Name:ARF4,Bovine ADP-ribosylation factor 4, ARF4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine ADP- ribosylation factor 4, ARF4 ELISA KIT allows for the in vitro quantitative determination of Bovine ADP-ribosylation factor 4, ARF4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine ADP- ribosylation factor 4, ARF4 ELISA KIT has been pre-coated with an Bovine ADP-ribosylation factor 4, ARF4 antibody specific to Bovine ADP-ribosylation factor 4, ARF4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine ADP-ribosylation factor 4, ARF4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine ADP-ribosylation factor 4, ARF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine ADP-ribosylation factor 4, ARF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Preparation of Myristoylated Arf1 and Arf6 Proteins
ADP-ribosylation factor (Arf) proteins were first identified as cofactors for cholera toxin-catalyzed ADP-ribosylation of the heterotrimeric G-protein Gs. Subsequent cloning led to the discovery that Arfs were part of a group of GTP-binding
ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
TheADP-ribosylation factor proteins (ARFs) are ~20-kDa guanine-nucleotidebinding proteins found in all eukaryotic cells, which are a critical part of the minimum machinery required for vesicle formation at the Golgi and other membranes (1 –
) M26066 2.8 +16 ADP-ribosylation factor 4 (ARF4) M36341.1 1.5 +116 Isoleucil-tRNA synthetase U04953
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