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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Platelet-activating factor acetylhydrolase IB subunit al
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Platelet- activating factor acetylhydrolase IB subunit alpha, PAFAH1B1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PAFAH1B1,PAFAHA,LIS1; LIS2; MDCR; MDS; PAFAH; LIS-1; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; PAFAH alpha; lissencephaly 1 protein; lissencephaly-1 protein; platelet-activating factor acetylhydrolase IB subunit alpha; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kD; platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 45kDa; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 45kDa
Search name
Bovine PAFAH1B1 ELISA KIT ,Bovine PAFAHA ELISA KIT ,Bovine LIS1 ELISA KIT ,Bovine LIS2 ELISA KIT ,Bovine MDCR ELISA KIT ,Bovine MDS ELISA KIT ,Bovine PAFAH ELISA KIT ,Bovine LIS-1 ELISA KIT ,Bovine PAF acetylhydrolase 45 kDa subunit ELISA KIT ,Bovine PAF-AH 45 kDa subunit ELISA KIT ,Bovine PAF-AH alpha ELISA KIT ,Bovine PAFAH alpha ELISA KIT ,Bovine lissencephaly 1 protein ELISA KIT ,Bovine lissencephaly-1 protein ELISA KIT ,Bovine platelet-activating factor acetylhydrolase IB subunit alpha ELISA KIT ,Bovine platelet-activating factor acetylhydrolase ELISA KIT ,Bovine isoform Ib ELISA KIT ,Bovine alpha subunit 45kD ELISA KIT ,Bovine platelet-activating factor acetylhydrolase isoform Ib subunit 1 45kDa ELISA KIT ,Bovine platelet-activating factor acetylhydrolase 1b regulatory subunit 1 45kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Platelet- activating factor acetylhydrolase IB subunit alpha, PAFAH1B1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAFAH1B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAFAH1B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAFAH1B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAFAH1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Human Plasma Platelet-Activating Factor Acetylhydrolase
The human plasma platelet-activating factor acetylhydrolase (PAF acetylhydrolase, l-alkyl-2-acetyl-glycerophosphocholine esterase, l-alkyl-2-acetyl- sn -glycero-3-phosphocholine acetohydrolase, EC 3.1.1.47) is a phospholipase A 2 activity
Measurement of Phosphoinositide 3-Kinase Activity
subunit. The class IB PI 3-kinases are stimulated by G protein αγ subunits and do not interact with the SH2-containing adaptors that bind class IA PI 3-kinases. Instead, the first identified member of this family p1 10γ, associates with a unique p101
Introduction of human 14-3-3 proteins
of Pseudomonas aeruginosa as does bovine brain factor activating exoenzyme S (FAS),which is itself a member of 14-3-3 proteins.In addition,GF14 omega binds calcium,as does the human 14-3-3 homologue reported to be a phospholipase A2.These results indicate
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