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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine SH2 domain-containing protein 1A,SH2D1A
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine SH2 domain- containing protein 1A, SH2D1A ELISA KIT
Product Name:Bovine SH2 domain- containing protein 1A, SH2D1A ELISA KIT
Packing:96T
Catalog No.:ELI-30370b
Gene Name:Bovine SH2 domain-containing protein 1A, SH2D1A
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine SH2 domain-containing protein 1A, SH2D1A
Alternative Name:SH2D1A,Bovine SH2 domain-containing protein 1A, SH2D1A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine SH2 domain- containing protein 1A, SH2D1A ELISA KIT allows for the in vitro quantitative determination of Bovine SH2 domain-containing protein 1A, SH2D1A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine SH2 domain- containing protein 1A, SH2D1A ELISA KIT has been pre-coated with an Bovine SH2 domain-containing protein 1A, SH2D1A antibody specific to Bovine SH2 domain-containing protein 1A, SH2D1A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine SH2 domain-containing protein 1A, SH2D1A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine SH2 domain-containing protein 1A, SH2D1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine SH2 domain-containing protein 1A, SH2D1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Figure 17.4.1 Photograph of a phage ELISA result. Using eight different clones that were isolated with GST‐SrcSH3 domain as the target, 25 µl of culture supernatant were added to pairs of microtiter plate wells containing GST‐SrcSH3 or GST
Procedures for In Vitro Production of Bovine Embryos
, Chenoweth PJ, Hansen PJ. Deleterious actions of gossypol on bovine spermatozoa, oocytes and embryos. Biol Reprod 1997; 57, 901-907.Edwards JL, Ealy AD, Monterroso VH, Hansen PJ. Ontogeny of temperature-regulated heat shock protein 70 synthesis
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
against macromolecular targets. They have proven particularly useful in profiling the binding specificity of protein modular domains (e.g., SH2 domains, BIR domains, and PDZ domains); subsequently, the specificity information can be used to predict the protein targets
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