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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Septin-7,SEPT7
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Septin- 7, SEPT7 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SEPT7,CDC10; CDC3; NBLA02942; SEPT7A; CDC10 (cell division cycle 10, S. cerevisiae, homolog); CDC10 protein homolog; septin-7; septin 7
Search name
Bovine SEPT7 ELISA KIT ,Bovine CDC10 ELISA KIT ,Bovine CDC3 ELISA KIT ,Bovine NBLA02942 ELISA KIT ,Bovine SEPT7A ELISA KIT ,Bovine CDC10 (cell division cycle 10 ELISA KIT ,Bovine S. cerevisiae ELISA KIT ,Bovine homolog) ELISA KIT ,Bovine CDC10 protein homolog ELISA KIT ,Bovine septin-7 ELISA KIT ,Bovine septin 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Septin- 7, SEPT7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEPT7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SEPT7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SEPT7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SEPT7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
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