Bovine Tyrosyl- tRNA synthetas

Bovine Tyrosyl- tRNA synthetase, cytoplasmic, YARS ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

YARS,CMTDIC; TYRRS; YRS; YTS; tyrosine tRNA ligase 1, cytoplasmic; tyrosine--tRNA ligase, cytoplasmic; tyrosyl--tRNA ligase; tyrosyl-tRNA synthetase, cytoplasmic; tyrosyl-tRNA synthetase

Search name

Bovine YARS ELISA KIT ,Bovine CMTDIC ELISA KIT ,Bovine TYRRS ELISA KIT ,Bovine YRS ELISA KIT ,Bovine YTS ELISA KIT ,Bovine tyrosine tRNA ligase 1 ELISA KIT ,Bovine  cytoplasmic ELISA KIT ,Bovine tyrosine--tRNA ligase ELISA KIT ,Bovine cytoplasmic ELISA KIT ,Bovine tyrosyl--tRNA ligase ELISA KIT ,Bovine tyrosyl-tRNA synthetase ELISA KIT , Bovine tyrosyl-tRNA synthetase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Tyrosyl- tRNA synthetase, cytoplasmic, YARS concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to YARS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for YARS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain YARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of YARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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