- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-26079b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Elongation factor 2,EEF2
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Elongation factor 2, EEF2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Elongation factor 2,EF-2, EEF2,eukaryotic translation elongation factor 2; elongation factor 2; EEF-2; EF-2; EF2; polypeptidyl-tRNA translocase
Search name
Bovine Elongation factor 2 ELISA KIT ,Bovine EF-2 ELISA KIT ,Bovine EEF2 ELISA KIT ,Bovine eukaryotic translation elongation factor 2 ELISA KIT ,Bovine elongation factor 2 ELISA KIT ,Bovine EEF-2 ELISA KIT ,Bovine EF-2 ELISA KIT ,Bovine EF2 ELISA KIT ,Bovine polypeptidyl-tRNA translocase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine elongation factor 2,EF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Support Protocol 3: Mouse Euthanasia and Tissue Dissection Support Protocol 4: Serum Insulin Quantification Using ELISA Support Protocol 5: Analysis of Human Cell Engraftment by Flow Cytometry
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