Bovine Autophagy protein 5, AT

Bovine Autophagy protein 5, ATG5 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ATG5, Autophagy protein 5,ATG5 autophagy related 5 homolog; APG5; APG5-LIKE; APG5L; ASP; hAPG5; apoptosis-specific protein; autophagy related 5

Search name

Bovine ATG5 ELISA KIT ,Bovine Autophagy protein 5 ELISA KIT ,Bovine ATG5 autophagy related 5 homolog ELISA KIT ,Bovine APG5 ELISA KIT ,Bovine APG5-LIKE ELISA KIT ,Bovine APG5L ELISA KIT ,Bovine ASP ELISA KIT ,Bovine hAPG5 ELISA KIT ,Bovine apoptosis-specific protein ELISA KIT ,Bovine autophagy related 5 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine Autophagy protein 5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy protein 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Autophagy protein 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Autophagy protein 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Autophagy protein 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.