- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-21763b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Protein phosphatase 1B,PPM1B
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Protein phosphatase 1B, PPM1B ELISA KIT
Product Name:Bovine Protein phosphatase 1B, PPM1B ELISA KIT
Packing:96T
Catalog No.:ELI-21763b
Gene Name:Bovine Protein phosphatase 1B, PPM1B
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Protein phosphatase 1B, PPM1B
Alternative Name:PPM1B,Bovine Protein phosphatase 1B, PPM1B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Protein phosphatase 1B, PPM1B ELISA KIT allows for the in vitro quantitative determination of Bovine Protein phosphatase 1B, PPM1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Protein phosphatase 1B, PPM1B ELISA KIT has been pre-coated with an Bovine Protein phosphatase 1B, PPM1B antibody specific to Bovine Protein phosphatase 1B, PPM1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Protein phosphatase 1B, PPM1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Protein phosphatase 1B, PPM1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Protein phosphatase 1B, PPM1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Substrate Tablete(antibody developed in goat) St. Louis,Mo 63178, USAAffinity isolated antibody adsorbed Lot # 043H6178With bovine horse and human serum protein Cat # 0h176Lot # 055H4835Lot # A-2179Tween 80Laboratories ,Detroit,Michigan ,USA1 drop of Tween
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
phosphatase‐conjugated secondary antibody Substrate 104 (or other alkaline phosphatase substrate; Sigma‐Aldrich) ELISA substrate
DETECTION OF RECOMBINANT PROTEINS BY ELISA
Refer to the Western Blot Protocol for preparation of bacterially expressed protein. From a 10 ml bacterial culture, 2-4 plates can be coated for a direct ELISA.Direct ELISA: Lyse and sonicate the bacteria that express the protein
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