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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Phenylethanolamine N-methyltransferase,PNMT
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Phenylethanolamine N- methyltransferase, PNMT ELISA KIT
Product Name:Bovine Phenylethanolamine N- methyltransferase, PNMT ELISA KIT
Packing:96T
Catalog No.:ELI-21711b
Gene Name:Bovine Phenylethanolamine N-methyltransferase, PNMT
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Phenylethanolamine N-methyltransferase, PNMT
Alternative Name:PNMT,Bovine Phenylethanolamine N-methyltransferase, PNMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Phenylethanolamine N- methyltransferase, PNMT ELISA KIT allows for the in vitro quantitative determination of Bovine Phenylethanolamine N-methyltransferase, PNMT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Phenylethanolamine N- methyltransferase, PNMT ELISA KIT has been pre-coated with an Bovine Phenylethanolamine N-methyltransferase, PNMT antibody specific to Bovine Phenylethanolamine N-methyltransferase, PNMT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Phenylethanolamine N-methyltransferase, PNMT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Phenylethanolamine N-methyltransferase, PNMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Phenylethanolamine N-methyltransferase, PNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phenylethanolamine N -methyltransferase (PNMT) (EC 2.1.1.28, S -adenosyl-L -methionine: phenylethanolamine N -methyltransferase) is the enzyme that catalyzes the biosynthesis of norepinephrine [noradrenaline; (NA)] to epinephrine [adrenaline
佚名 肾上腺髓质嗜铬细胞分泌肾上腺素(epinephrine,E)和去甲肾上腺素(norepinephrine,NE)都是儿茶酚胺激素。 (一)髓质激素的合成与代谢 髓质激素的合成与交感神经节后纤维合成去甲肾上腺素的过程基本一致,不同的是在嗜铬细胞胞浆中存在大量的苯乙醇胺氮位甲基移位酶(phenylethanolamine-N
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
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