- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-20292b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Ribonuclease H2 subunit B,RNASEH2B
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT
Product Name:Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT
Packing:96T
Catalog No.:ELI-20292b
Gene Name:Bovine Ribonuclease H2 subunit B, RNASEH2B
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Ribonuclease H2 subunit B, RNASEH2B
Alternative Name:RNASEH2B,Bovine Ribonuclease H2 subunit B, RNASEH2B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT allows for the in vitro quantitative determination of Bovine Ribonuclease H2 subunit B, RNASEH2B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT has been pre-coated with an Bovine Ribonuclease H2 subunit B, RNASEH2B antibody specific to Bovine Ribonuclease H2 subunit B, RNASEH2B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ribonuclease H2 subunit B, RNASEH2B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ribonuclease H2 subunit B, RNASEH2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ribonuclease H2 subunit B, RNASEH2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(E2758) BME amino acids solution (B6766) Bovine serum albumin, Fraction V (A3311) Bovine serum albumin, essentially fatty acid free (A6003) Calcium chloride (C7902) Epinephrine (E4250) DL-Lactic acid, sodium salt, 98% syrup (L-
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
in the top right and bottom right corner (A12, B12, G12, H12), the 25 pg/well standard next to the 100 pg standard (A2, B2, G2, H2), and the 12.5 pg/well standard next to the 50 pg standard (A11, B11, G11, H11). The lower concentrations are run in quadruplicate
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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