- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-14833b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine RNA-binding Raly-like protein,RALYL
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine RNA- binding Raly- like protein, RALYL ELISA KIT
Product Name:Bovine RNA- binding Raly- like protein, RALYL ELISA KIT
Packing:96T
Catalog No.:ELI-14833b
Gene Name:Bovine RNA-binding Raly-like protein, RALYL
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine RNA-binding Raly-like protein, RALYL
Alternative Name:RALYL,Bovine RNA-binding Raly-like protein, RALYL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine RNA- binding Raly- like protein, RALYL ELISA KIT allows for the in vitro quantitative determination of Bovine RNA-binding Raly-like protein, RALYL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine RNA- binding Raly- like protein, RALYL ELISA KIT has been pre-coated with an Bovine RNA-binding Raly-like protein, RALYL antibody specific to Bovine RNA-binding Raly-like protein, RALYL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine RNA-binding Raly-like protein, RALYL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine RNA-binding Raly-like protein, RALYL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine RNA-binding Raly-like protein, RALYL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
【资源】[共享]Adhesion Protein Protocols——（很有用的）
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哺乳动物RNAi技术 Mammalian RNA Interference
are less effectively targeted by siRNAs, as these may be richer in regulatory protein-binding sites. UTR-binding proteins and/or translation initiation complexes could interfere with binding of RISC to the target RNA. If the intent
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